Analysis of biomolecules B-BMOL.003
Laboratorium (LB) Semestr zimowy 2024/2025

Selected methods of protein analysis:

1) Manufactures manuals:

• https://nanotempertech.com/ - Bio-layer interferometry (BLI),

• https://nanotempertech.com/ - Stability profile - Prometeus,

• https://www.sartorius.com/ - Microscale thermophoresis (MST),

2) Online materials indicated by the teacher

Analysis of biomolecules using spectroscopic methods (UV-Vis, FTIR, Raman spectroscopy):

Lakowicz J.R. (ed.) Principles of Fluorescence Spectroscopy, Springer, 3rd ed. 2006. Corr. 5th printing 2010

2) Gryczynski K.Z. Practical Fluorescence Spectroscopy, Taylor & Francis Inc

3) laboratory manual prepared for the course by the teacher

Mass Spectrometry and Nuclear Magnetic Resonance spectroscopy in analysis of biomolecules:

Bibliography:

1) Theoretical foundations of mass spectrometry (MS) and Nuclear Magnetic Resonance spectroscopy (NMR) (multimedia presentations) and laboratory manual prepared by the teachers

2) Online materials indicated by the teachers

Assessment methods and criteria:

controlling the accuracy of an experiment and correctness of the report, laboratory report

Three course blocks:

Selected methods of protein analysis

Analysis of biomolecules using spectroscopic methods (UV-Vis, FTIR, Raman spectroscopy)

Mass Spectrometry and Nuclear Magnetic Resonance spectroscopy in analysis of biomolecules

Teaching methods:

labs, experiment, group work, discussion and interpretation of results as well as individual student reports from laboratory exercises