Advanced biochemistry

Basic knowledge of biochemistry

Contact hours with the teacher: 60 (2.5 ETCS)

Lectures - 20 h

Laboratories - 40 h

Non-contact hours: 60 h (2.5 ECTS)

- preparation for the exam: 30 h

- preparation for labs: 20 h

- preparation of reports from laboratory exercises: 5 h

- literature study: 5 h

Total amount of student's working hours: 120

Amount of ECTS points: 5

Methods of verification of learning outcomes for first degree programs approved on the basis of Resolution of the Senate of the UMCS No.

XXIV-27.18/19 of May 29, 2019. (i.e., from the 2019/2020 educational cycle):

W1 - W3 - lecture written exam

W4 - W5 - laboratory - oral question, written colloquia, reports on exercises performed

U1 - U4 - laboratory - oral question, written colloquia, reports on exercises performed

K1 - lecture written exam

K2 - laboratory - oral question, written colloquia, reports from performed exercises

Lectures: 20 h (10 meetings, 2 x 45 min.)

1. Nucleic acids: structure, function, biosynthesis.

2. Protein:synthesis, folding, degradation and turnover.

3. Regulation of transcription and translation.

4 - 5. Functional roles of protein (hemoglobin, myoglobin, actin, myosin, molecular motors).

6 - 7. Pathways of aminoacids biosynthesis and degradation.

8. Overview of nitrogen metabolism.

9. Biosynthesis and degradation of polysachrides.

10. Lipid metabolism: cholesterol and plasma lipoprotein.

Laboratories: 40 h (8 x 5h)

The laboratory covers topics related to practical aspects of preparation and analysis of biological material of plant and fungal origin and the application of chromatographic, spectrophotometric and electrophoretic techniques in biochemical analyzes of bioactive compounds: organic extraction of alkaloids, isolation of peptides from cell hydrolyzates, protein purification and electrophoresis, detection of enzymatic activities, purification and identification of amino acids, adsorption chromatography.

Berg J.M, Tymoczko J.L., Gatto G., Stryer L., Biochemistry, New York: W.H. Freeman and Company, 2015;

Voet D., Judith G. Voet, Biochemistry, 4th Edition, 2010;

Voet D.J., Voet J.G., Pratt C.W. Principles of Biochemistry 5th ed. John Wiley &Sons, Inc. 2018;

Clark D., Pazdernik N, McGehee M., Molecular Biology. Academic Cell. Elsevier 2019;

Cooper G.M The Cell Molecular Approach, Oxford University Press, 2019

The Encyclopedia of Biochemistry, 1967, (R.J. Williams and E.M. Lansford, Jr., Ed.), Reinhold Publishing Corporation, New York

Matsuura, H.N., Fett-Neto, A.G. (2015). Plant Alkaloids: Main Features, Toxicity, and Mechanisms of Action. In: Gopalakrishnakone, P., Carlini, C., Ligabue-Braun, R. (eds) Plant Toxins. Toxinology. Springer, Dordrecht.

Bańkowski E. (Ed.) 2013 Biochemistry workbook. Medical University of Białystok.

Bisswanger H. 2011. Practical enzymology. Willey-Blackwell.

Walker, JM (Ed) 2002 The protein protocols Handbook. 2nd Edition, Humana Press, Totowa, New Jersey

articles from current scientific literature

Pursuant to the Resolution of the Senate of the UMCS by Resolution No. XXIV-27.18/19 dated May 29, 2019, i.e., from the 2019/2020 education cycle

Student:

KNOWLEDGE

W1 - Defines and describes in depth biochemical molecules of different complexity, K_W01.

W2 - Distinguishes, characterizes in depth the biochemical processes occurring in the body and explains the most important pathways of indirect and secondary metabolism, K_W02, K_W03

W3 - Knows the pathways and locations of indirect and secondary metabolism and the basis of its integration and regulation, K_W02

W4 - Understands the importance of inference based on experiment, K_W04

W5 - Knows and understands the principles of planning and conducting biochemical experiments, knows the principles of safe laboratory work, K_W04

SKILLS

U1 - Can select and use appropriate methods to isolate biologically active compounds, K_U02

U2 - Can select and apply appropriate methods for determination and analysis of biologically active compounds, K_U02

U3 -Able to present and interpret the results of conducted experiments using advanced information and communication techniques, K_U03, K_U04

U4 -Uses the acquired knowledge and skills in practice, K_U03, K_U05

SOCIAL COMPETENCES

K1 -Understands the need for an active attitude in acquiring, completing and updating expanded biochemical knowledge for scientific and professional development, K_K03

K2 - Student is responsible for his own safety and that of his surroundings in experimental work with chemicals, biological material and specialized apparatus, K_K01