Laboratory techniques
Informacje ogólne
Kod przedmiotu: | B-BT.033E |
Kod Erasmus / ISCED: | (brak danych) / (brak danych) |
Nazwa przedmiotu: | Laboratory techniques |
Jednostka: | Wydział Biologii i Biotechnologii |
Grupy: | |
Punkty ECTS i inne: |
(brak)
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Język prowadzenia: | angielski |
Wymagania wstępne: | (tylko po angielsku) Completed course of organic chemistry and analytical chemistry |
Godzinowe ekwiwalenty punktów ECTS: | (tylko po angielsku) Amount of ECTS points: 6.5 Contact hours with the teacher: - laboratories - 60 h Non-contact hours: 60 h - preparation for tests: 20 h - preparation for labs: 15 h - preparation of reports from laboratory exercises: 15 h - literature study: 10 h |
Sposób weryfikacji efektów kształcenia: | (tylko po angielsku) Method of verifying the learning outcomes of the first-cycle studies approved on the basis of Resolution of the Senate of Maria Curie-Skłodowska University No. XXII-39.6 / 12 of April 25, 2012, i.e. from the 2012/2013 education cycle: laboratory - developing exercises and colloquia during the semester (W1-W6, U1-U7, K1-K4) Method of verifying the learning outcomes of the first-cycle studies approved on the basis of the Resolution of the Senate of Maria Curie-Skłodowska University No. XXIV-27.18 / 19 of May 29, 2019, i.e. from the 2019/2020 education cycle: laboratory - developing exercises and colloquia during the semester (W1-W5, U1-U4, K1-K2) |
Pełny opis: |
(tylko po angielsku) Part I (Dept. Molecular Biology) 1. Preparation of buffer solutions 2. Quantitative determination of protein 3. Cell fractionation (cell disintegration, differential and density gradient centrifugation, salting out and protein dialysis) 4. Protein chromatography 5. Electrophoretic protein separation techniques 6. Immunodetection of proteins Part II (Dept. Genetics and Microbiology) 1. UV-Vis spectrophotometry and statistical evaluation of results. 2. Refractometry and polarimetry. 3. Chromatography - part 1. Qualitative analysis. 4. Chromatography - part 2. Quantitative analysis. 5. Mass spectrometry (part I and II) 6. Nuclear magnetic resonance spectroscopy (NMR). |
Literatura: |
(tylko po angielsku) Part I Script for exercises in laboratory techniques Part II Descriptions of practical part and materials from teacher (classes II 1-7). |
Efekty uczenia się: |
(tylko po angielsku) Based on the Resolution of the UMCS Senate No. XXII-39.6 / 12 of April 25, 2012, i.e. from the 2012/2013 education cycle: KNOWLEDGE W1. Understands the basic processes occurring in living organisms at the molecular level. K_W01 W2. Knows the basic concepts and terminology used in natural sciences. K_W05 W3. Has knowledge of the development of knowledge, especially in the field of experimental biology, and the related improvement of research techniques. K_W06 W4. Understands the importance of mathematical description and statistical interpretation of results for the characteristics of processes at different levels of the organization of the living world. K_W13 W5. Knows the basic research and laboratory tools and techniques used in biological sciences. K_W17 W6. Describes the processes occurring in living organisms based on experiments and observations, and based on data from scientific sources. K_W11 SKILLS U1. Uses basic research and laboratory tools and techniques in the field of biological sciences. K_U01 U2. Performs simple analyzes of biological material, performs assessments and diagnoses, plans simple analytical and preparative procedures. K_U02 U3. Is able to perform basic experiments in biophysics, chemistry, molecular biology and biochemistry in the laboratory. K_U04 U4. Uses mathematical and statistical methods to describe phenomena, analyze the experiment and process the results. K_U05 U5. Prepares written reports on performed experiments. K_U15 U6. Uses information technology to describe and interpret biological processes. K_U06 U7. He understands selected fragments of specialized scientific texts. K_U09 SOCIAL COMPETENCE K1. Demonstrates an active attitude in acquiring, supplementing and updating biological knowledge. K_K03 K2. Able to work in a group to solve problems together and efficiently perform specific tasks. K_K08 K3. He analyzes the tasks commissioned for implementation in terms of their correct and efficient performance, determining the order of activities and agreeing on the principles of cooperation in the group. K_K09 K4. Is aware of the responsibility for own safety and the environment in experimental work with apparatus, chemicals and biological material. K_K10 On the basis of Resolution of the Senate of Maria Curie-Skłodowska University No. XXIV-27.18 / 19 of May 29, 2019, i.e. from the training cycle 2019/2020 KNOWLEDGE W1. The graduate knows and understands at an advanced level selected facts, concepts, objects, phenomena and the complex relationships between them and theories explaining them, constituting the basic general knowledge in the field of scientific disciplines, mainly exact and natural sciences, forming the theoretical basis of biology. K_W01 W2. The graduate knows and understands the basic research and laboratory methods and techniques used in modern biology. K_W02 W3. The graduate knows and understands at an advanced level the relationship between biology and other natural disciplines, enabling the interpretation and generalization of knowledge. K_W03 W4. The graduate knows and understands at an advanced level biology-specific selected issues of advanced detailed knowledge, including basic processes occurring at the molecular and cellular level. K_W04 W5. The graduate knows and understands the principles of mathematical description, IT techniques and statistical interpretation of results as well as their importance for the characteristics of phenomena and processes at various levels of the organization of the living world K_W07 SKILLS U1. The graduate is able to properly select and use appropriate research methods and tools, and present the results of experiments or observations and conclusions, including analysis of professional literature, in written and oral form, using advanced information and communication techniques. K_U03 U2. The graduate is able to carry out experiments, observations and measurements using appropriate research and laboratory tools and methods, as well as interpret the results obtained and draw conclusions based on his knowledge. K_U04 U3. The graduate is able to communicate with the environment using specialized terminology in the field of biology and related natural sciences. K_U05 U4. The graduate is able to plan and organize individual and team work in order to efficiently solve problems and perform specific tasks. K_U07 SOCIAL COMPETENCE K1. The graduate is ready to critically assess his knowledge and received content, and to recognize the importance of general and specialist knowledge in the field of biology in solving cognitive and practical problems, as well as to seek expert opinions in the event of difficulties in solving problems by himself. K_K02 K2. The graduate is ready to take care of the achievements and traditions of the profession of biologist K_K06 |
Właścicielem praw autorskich jest Uniwersytet Marii Curie-Skłodowskiej w Lublinie.