Medical Microbiology

Course description: The course provides a basic theoretical and technical study of the structure, molecular biology, pathogenesis, epidemiology, and laboratory identification of the various bacteria that cause human diseases.

Lecture:

Introduction to Medical Microbiology:

Normal Flora (human microbiome)

Antimicrobial Chemotherapy

Gram-positive cocci: Staphylococcus, Streptococcus,

Gram-positive spore-formers: Bacillus, Clostridium,

Gram-negative bacilli: Enterobacterales; Yersinia, Salmonella, Shigella, Escherichia, Klebsiella, Proteus,

Gram-negative bacilli oxidase positive Pseudomonas, Burkholderia,

Gram-negative curved bacilli: Vibrio, Campylobacter, Helicobacter,

Gram-negative coccobacilli: Brucella, Bordetella

Fastidious Gram-negative bacteria: Neisseria, Haemophilus,

Legionella,

Animal-associated bacteria: Francisella, Pasteurella,

Spirochetes: Treponema, Borrelia,

Gram-positive bacilli: Listeria, Corynebacteria, Mycobacteria,

Obligate intracellular bacteria: Rickettsia, Chlamydia,

Cell Wall-less bacteria: Mycoplasma,

Laboratory:

Isolation and culturing of bacteria. Microscopic examination of bacterial morphology by different staining methods (Gram, Neisser, Ziehl-Neelsen). Examination and identification of selected groups of pathogenic bacteria by using laboratory detection methods: molecular, morphological, immunological and cultural. Detection of antibiotic resistance mechanisms. Biochemical test for identification of bacteria. Determination of MIC and MBC. PCR methods for identification of pathogenic strains.

Identification and differentiation of Staphylococcus and Micrococcus spp.

Characteristics of Streptococcus spp.

Identification of Moraxella spp. and Haemophilus spp.

Detection methods for Pseudomonas spp.

Isolation and identification of Enterobacteriaceae (Escherichia, Enterobacter, Serratia)

Microbiological diagnosis of urinary tract infection

Characteristics and identification of Yersinia and Klebsiella strains

Biochemical and serological identification of Salmonella spp.

Isolation and identification of Shigella spp.

Identification of Corynebacterium species

Diagnosis of Listeria monocytogenes and Erysipelothrix rhusiopathiae

Detection and identification of Mycobacterium species

DNA isolation and detection of Staphylococcus aureus and Listeria monocytogenes by polymerase chain reaction amplification of the mec gene and hly, respectively (two exercises)

Summary of information on specific groups of pathogenic bacteria

1. 1. Harrison’s Infectious Diseases. by Dennis L. Kasper, Anthony S. Fauci, 2010.

2. Foundations in microbiology: Kathleen Park Talaro, McGraw-Hill education - europe, NY, 2008

3. Microbiology with diseases by body system. 4th ed. By R.W. Bauman, 2017

4. Medical Microbiology by F.H. Kayzer, K.A. Bienz, J. Eckert, R.M. Zinkernagel, 2004

5. Practical Manual of Medical Microbiology by C.P. Prince, 2008

6. Microbiology with Diseases by Taxonomy by R.W. Bauman, Pearson, 2011

Learning outcomes:

KW_01: Recognises basic processes occurring in living organisms and the basic etiological factors of human bacterial infective diseases

KW_02: Describes the relationships between host organisms and microorganisms

KW_05: Knows basic notions and terminology used in natural sciences and refers them to the conceptual categories of the philosophy of nature

KW_08: Acknowledges the importance of conservation of biodiversity as a source of biological material for practical applications

KW_17: Knows basic laboratory and field research tools and techniques applied in biological sciences

KW_18: Knows the basics of management of occupational health and safety in Poland

KW_19: Identifies hazards associated with work with chemicals and biological material and methods for prevention of risk as well as first-aid principles

Skills:

K_U01: Uses basic laboratory and field research tools and techniques applied in biology sciences

K_U02: Performs simple analyses of biological material, evaluation and diagnoses, and designs simple analytical and preparatory procedures

K_U04: Has an ability to conduct basic laboratory experiments in biophysics, chemistry, biochemistry, and biology and capability to work as a scientist in a research or diagnostic microbiology laboratory

K_U11: Has an ability to use various sources of information, including university library resources and academic and scientific internet websites

Social competence:

K_K01: Is able to assess knowledge and skills acquired at the undergraduate level and recognises the need for continuous improvement of language skills and extending specialist and general knowledge

K_K02: Acknowledges the necessity of upgrading occupational competencies

K_K04: Chooses specialisation subjects required for future scientific career or other jobs applying the obtained knowledge for diagnostics and antimicrobial proceeding of bacteria that cause disease in humans

K_K08: Has an ability to work in a team in order to solve problems, fulfil tasks efficiently, and prepares presentations

K_K10: Acknowledges responsibility for own safety and safety of the environment during experimental work with apparatus, chemicals, and biological material