Microbial infectivity, drug resistance and diagnostics

The student should have basic knowledge of microbiology

The student should have basic skills for working in a microbiology laboratory

Amount of ECTS points: 3.0

Contact hours with the teacher: 30 (1.5 ETCS)

Lectures - 15 h

Laboratories - 15 h

Non-contact hours: 30 h (1.5 ECTS)

- preparation for the exam: 15 h

- preparation for labs: 10 h

- preparation of reports from laboratory exercises: 2.5 h

- literature study: 2.5 h

lecture - written test at the end of course (W1-W3, U2-U4, K1-K3)

laboratory - developing exercises

The lecture aims to learn about the classification and mechanisms of action of antibiotics and the methods used to determine antibiotic susceptibility. Mechanisms of antimicrobial resistance of microorganisms and the problem of bacterial multidrug resistance (alarm pathogens) will be discussed. Application of molecular methods for typing bacteria and detection of antibiotic resistance genes and genes encoding virulence factors. Familiarity with serological methods in the diagnosis of pathogenic bacteria, yeasts and fungi

Laboratory

Determination of antibiotic susceptibility (disk-diffusion method, MIC determination, E-tests), phenotypic methods for determining mechanisms of drug resistance. Detection of genes encoding virulence factors (surface antigens and toxins) by PCR; typing of strains by BOX-PCR technique. Serotyping of selected microorganisms using latex tests and antisera

Materials form teachers

Antibiotic Drug Resistance, Editor(s):José-Luis Capelo-Martínez, Gilberto Igrejas

Print ISBN:9781119282525 |Online ISBN:9781119282549 John Wiley & Sons, Inc. 2020

Bacterial Pathogens and Their Virulence Factors; Douglas I. Johnson

Online ISBN 978-3-319-67651-7 Springer, Cham 2018

Sherris Medical Microbiology 7e Editor Kenneth J. Ryan MD ISBN 978-1-259-85980-9 McGraw Hill 2017

Jawetz, Melnick, & Adelberg’s Medical Microbiology, 28e

Editors Stefan Riedel, et al. McGraw Hill; 2019

1. KNOWLEDGE:

W1. The graduate has in-depth knowledge of classification and action of antibiotics, mechanisms of drug resistance, methods of determining drug susceptibility of microorganisms. He is familiar with techniques for detection of antibiotic resistance genes and those coding for virulence factors, as well as strain typing by BOX-PCR technique.Has advanced knowledge of serological and molecular methods used in the diagnosis of pathogenic bacteria, yeasts and fungi. K_W01, K_W02, K_W03

W2. The graduate knows and understands the basics of methodology used in natural sciences, the principles of planning and conducting biological experiments or observations, and rules of formulation of appropriate conclusions describing and interpreting natural phenomena and processes. K_W04

W3. The graduate has profound knowledge and understanding of the specificity of biology as well as the main development trends and the latest achievements of this science, including significant accomplishments for the human, economy, and environment . K_W05

2. SKILLS:

U1. The graduate is able to formulate and solve research problems on drug resistance, serotyping, virulence of microorganisms based on the acquired knowledge, formulate correct conclusions and innovatively solve a variety of molecular biology tasks in unpredictable conditions. K_U01

U2. Is able to properly select and use sources of scientific information, critically evaluate and analyze data and synthesize and creatively interpret them. K_U02

U3. Graduates are able to work individually, cooperate with other researchers as part of teamwork, take a leadership role in teams, and direct the work of a team in the design and implementation of various tasks in the field of biotechnology. K_U09

3. SOCIAL COMPETENCE:

K1. The graduate is ready to design and develop patterns of proper conduct in the work and life environment, take the initiative, and critically assess himself/herself as well as teams and organizations to which he/she belongs. K_K01

K2. The graduate is ready to be the head of a team and take responsibility for the team and the decisions made. K_K02

K3. The graduate is ready to assess his/her knowledge and acquired information critically, recognize the importance of biological knowledge in solving theoretical and practical problems, and seek expert opinions in the case of difficulties in solving problems independently. K_K03