Animal and plant cell and tissue in vitro cultures

Animal part:

Theoretical basics of cell cultures. Organization and equipment of cell culture laboratory. Cell culture conditions and requirements; culture media. Animal and human cell culture handling. Cell culture derivation, handling of the primary cell lines. Methods used in the study of cell biology at cellular, subcellular and molecular levels.

Plant part:

Theoretical basics of plant cell and tissue cultures. Plant cell and tissue culture laboratory – basic equipment and organization of work. Culture media preparation. Main techniques of sterilization and preparation of plant material. Initiation of various types of plant cultures in in vitro conditions.Initiation and establishment of callus culture from different types of explants. Induction of organogenesis, embryogenesis and plant regeneration from cultured explants. Plant micropropagation methods.Induced androgenesis and gynogenesis as methods of haploid plants production.

Animal part:

1. Verma AS, Singh A. 2020. Animal Biotechnology. Models in Discovery and Translation (2nd ed.). Elsevier Inc.: Chapter 14 - Animal tissue culture principles and applications (Verma A, Verma M, Singh A, p. 269-293).

2. Vunjak-Novakovic G, Freshney IR. 2006. Culture of Cells for Tissue Engineering (1st ed.). John Wiley & Sons: Chapter 1 – Basic Principles of Cell Culture (Freshney IR, p. 3-22).

3. Wilson K, Walker J. 2010. Principles and Techniques of Biochemistry and Molecular Biology (7th ed.). Cambridge University Press.

4. Koledova Z. 2017. 3D Cell Culture. Methods and Protocols. Humana New York.

5. Vikas B, Fasullo M. 2019. Cell Growth. IntechOpen, London: Chapter 7 – Cell-Based Assays in Cancer Research (Vikas B, Sukumaran A).

6. Larramendy ML, Soloneski S. 2018. Genotoxicity - A Predictable Risk to Our Actual World. IntechOpen, London: Chapter 1 – In Vitro Cytotoxicity and Cell Viability Assays: Principles, Advantages, and Disadvantages (Aslantürk ÖS).

7. McCoy Jr, JP. 2002. Basic principles of flow cytometry. Hematology/Oncology Clinics of North America 16, p. 229-243.

8. Katt ME, Placone AL, Wong AD, Xu ZS Searson PC. 2016. In Vitro Tumor Models: Advantages, Disadvantages, Variables, and Selecting the Right Platform. Frontiers in Bioengineering and Biotechnology 4: 12.

Plant part:

1. Bhatia S, Sharma K, Dahiya R, Bera T. 2015. Modern Applications of Plant Biotechnology in Pharmaceutical Sciences (1st ed.). Elsevier Inc.

2. Borém A, Fritsche-Neto R. 2014. Biotechnology and Plant Breeding. Applications and Approaches for Developing Improved Cultivars (1st ed.). Elsevier Inc.: Chapter 7 - Tissue Culture Applications for the Genetic Improvement of Plants (Pasqual M, Rodrigues Soares J D, Almendagna Rodrigues F); Chapter 9 - Double Haploids (Fritsche-Neto R, Domingos Garbuglio D, Borém A).

3. Chandra S, Lata H, Varma A. 2013. Biotechnology for Medicinal Plants. Micropropagation and Improvement (1st ed.). Springer, Berlin, Heidelberg: Chapter 6 - Epilobium Sp. (Willow Herb): Micropropagation and production of secondary metabolites (Constantin D, Coste A, Mircea T); Chapter 8 - Advances in microspore culture technology: a biotechnological tool for the improvement of medicinal plants (Ferrie AMR); Chapter 9 - Implications of cellular heterogeneity on plant cell culture performance (Patil RA, Roberts SC).

4. Smith RH. 2013. Plant Tissue Culture. Techniques and Experiments (3rd ed.). Elsevier Inc.

5. Altman A, Hasegawa PM. 2012. Plant Biotechnology and Agriculture. Prospects for the 21st Century. (1st ed.). Elsevier Inc.: Chapter 15 - An engineering view to micropropagation and generation of true to type and pathogen-free plants (Khayat E); Chapter 30 - Plants as factories for bioplastics and other novel biomaterials (van Beilen JB, Poirier Y).

6. Loyola-Vargas VM, Ochoa-Alejo N. 2012. Plant Cell Culture Protocols (3rd ed.). Springer, Berlin, Heidelberg.

7. Imani J, Kumar A, Neumann K. 2009. Plant Cell and Tissue Culture - A Tool in Biotechnology. Basics and Application (1st ed.). Springer, Berlin, Heidelberg.

On the basis of Appendix 6 to MCSU Senate Resolution No XXIV-27.18/19 published 29.05.2019:

Animal part:

Knowledge:

W1. The graduate knows and understands basics and advanced issues related to cell biology at cellular, subcellular and molecular levels (K_W01-03).

W2. The graduate knows and understands the theoretical basis of methods used in handling of animal and human cell in vitro cultures (K_W03-04).

W3. The graduate knows and understands the methods used to analyze cell biology and impact of exogenous agents on it (K_W04-05).

W4. The graduate knows and understands the need to use in vitro animal cell cultures and its impact on modern science (K_W06-07).

Skills:

U1. The graduate is able to perform procedures in aseptic conditions (K_U02).

U2. The graduate is able to handle animal cell cultures (K_U02, K_U05).

U3. The graduate is able to perform analytical methods on cell cultures at cellular and molecular level (K_U04, K_U05).

U4. The graduate is able to analyze and interpret results obtained from scientific methods carried on cell cultures (K_U05).

Social competence:

K1. The graduate is ready to design experiments using in vitro cell cultures (K_K01).

K2. The graduate is ready to verify their knowledge, solve biological problems related to the cell cultures and share the knowledge (K_K03, K_K04).

Plant part:

Knowledge:

W1. W1. The graduate knows the principles of preparing sterile media, multiplying plant cell mass and conducting various types of in vitro cultures while maintaining sterility and occupational safety (K_K01, K_K02).

W2. The graduate knows the principles of directed tissue and cell culture using in vitro culture methods (K_K03, K_K04).

W3. The graduate knows and understands the need to use in vitro plant cell and tissue cultures and its impact on modern science (K_K06).

Skills:

U1. The graduate is able to prepare sterile media for plant tissue cultures (K_U02).

U2. The graduate is able to perform directed regeneration of plants from primary explants (K_U02, K_U03).

U2. The graduate is able to initiate and conduct cultures of plant organs, callus, and generative parts of plants (K_U05)

Social competence:

K1. The graduate is ready to disseminate the model of proper conduct in the in vitro plant tissue culture laboratory and independently undertakes a critical assessment of the correctness of action in solving a research problem (K_K01).

K2. The graduate is ready to notice social and ethical problems resulting from the implementation of tissue culture methods (K_K03).